174. Mediu pentru cultivarea celulelor

(D-MEM F-12 Medium (ATCC 30-2006), sau echivalent minim 500 ml Conține: Săruri anorganice (g/litru) - CaCl2 (anhydrous) 0.11665; CuSO4 (anhydrous) 0.0000008; Fe(NO3)3•9H2O 0.00005; FeSO4•7H2O 0.000417; MgSO4 (anhydrous) 0.08495; KCl 0.3118; NaCl 7.00000; NaHCO3 1.20000 (hemicalcium); Na2HPO4 (anhydrous) 0.07100; NaH2PO4 •H2O 0.06250; ZnSO4•7H2O 0.000432. Vitamine (g/litru)- D-Biotin 0.00000365; Choline Chloride 0.00898; Folic Acid 0.00265; myo-Inositol 0.01261; Niacinamide 0.00202; D-Pantothenic Acid 0.00224); Pyridoxine•HCl 0.00203; Riboflavin 0.00022; Thiamine•HCl 0.00217; Vitamin B-12 0.00068.
Amino Acizi (g/litru) - L-Alanine 0.00445; L-Arginine•HCl 0.14750 ; L-Asparagine•H2O 0.00750; L-Aspartic Acid 0.00665; L Cysteine•HCl•H2O 0.01756 ; L-Cystine•2HCl 0.03129 ; L-Glutamic Acid 0.00735; L-Glutamine 0.36510; Glycine 0.01875; L-Histidine•HCl•H2O 0.03148; L-Isoleucine 0.05437; L-Leucine 0.05895; L-Lysine•HCl 0.09135; L-Methionine 0.01724; L-Phenylalanine 0.03548; L-Proline 0.01725; L-Serine 0.02625; L-Threonine 0.05355; L-Tryptophan 0.00902; L-Tyrosine•2Na•2H2O 0.05582; L-Valine 0.05285.Alte componente (g/litru)- D-Glucose 3.15100; HEPES 3.57480; Hypoxanthine 0.00239; Linoleic Acid 0.000044; Phenol Red, Sodium Salt 0.00810; Putrescine•2HCl 0.00008; Pyruvic Acid•Na 0.05500; DL-Thioctic Acid 0.000105; Thymidine 0.000365. Certificat de analiză.