238. Mediu pentru cultivarea celulelor (D-MEM F-12 Medium (ATCC® 30-2006™), sau echivalent minim 500 ml

Conține: Săruri anorganice (g/litru) - CaCl2 (anhydrous) 0.11665; CuSO4 (anhydrous) 0.0000008; Fe(NO3)3·9H2O 0.00005; FeSO4·7H2O 0.000417; MgSO4 (anhydrous) 0.08495; KCl 0.3118; NaCl 7.00000; NaHCO3 1.20000 (hemicalcium); Na2HPO4 (anhydrous) 0.07100; NaH2PO4 ·H2O 0.06250; ZnSO4·7H2O 0.000432.
Vitamine (g/litru)- D-Biotin 0.00000365; Choline Chloride 0.00898; Folic Acid 0.00265; myo-Inositol 0.01261; Niacinamide 0.00202; D-Pantothenic Acid 0.00224); Pyridoxine·HCl 0.00203; Riboflavin 0.00022; Thiamine·HCl 0.00217; Vitamin B-12 0.00068.
Amino Acizi (g/litru) - L-Alanine 0.00445; L-Arginine·HCl 0.14750 ; L-Asparagine·H2O 0.00750; L-Aspartic Acid 0.00665; L-Cysteine·HCl·H2O 0.01756 ; L-Cystine·2HCl 0.03129 ; L-Glutamic Acid 0.00735; L-Glutamine 0.36510; Glycine 0.01875; L-Histidine·HCl·H2O 0.03148; L-Isoleucine 0.05437; L-Leucine 0.05895; L-Lysine·HCl 0.09135; L-Methionine 0.01724; L-Phenylalanine 0.03548; L-Proline 0.01725; L-Serine 0.02625; L-Threonine 0.05355; L-Tryptophan 0.00902; L-Tyrosine·2Na·2H2O 0.05582; L-Valine 0.05285.
Alte componente (g/litru)- D-Glucose 3.15100; HEPES 3.57480; Hypoxanthine 0.00239; Linoleic Acid 0.000044; Phenol Red, Sodium Salt 0.00810; Putrescine·2HCl 0.00008; Pyruvic Acid·Na 0.05500; DL-Thioctic Acid 0.000105; Thymidine 0.000365. Certificat de analiză.